RV IgG Rapid Test Uncut Sheet

RV IgG Rapid Test

Type: Uncut Sheet

Brand: Bio-mapper

Catalog:RT0521

Specimen:WB/S/P

Sensitivity:91.10%

Specificity:99%

Rubella mostly occurs in school-age children and adolescents, and more than 80% of the population is positive for this virus antibody. Pregnant women were infected with rubella virus before 20 weeks of pregnancy, and the incidence of fetal teratogenesis was high. Adults and children infected with rubella virus can cause skin rashes. ELISA: The carrier coated with inactivated rubella virus antigen can bind with specific antibody in the tested sample, and the corresponding antibody is detected with enzyme labeled anti human immunoglobulin and substrate.


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(1) For sample collection and screening test, only a single blood sample needs to be collected. However, if it is necessary to judge the immune status of people infected with the virus, it is necessary to take samples from suspected rubella patients within 3 days after the onset of the rash and the following 14 to 21 days for simultaneous detection.
(2) The same as the general ELISA, add PBS 50 in each hole of the control and sample μ l. Continue adding sample 10 μ l. Heat at 25 ℃ for 45 min, wash and dry.
(3) Add enzyme markers to each well 250 μ l. The same method is used for heat preservation and washing.
(4) Add pNPP substrate solution 250 μ l. After heat preservation and washing by the same method, add 1mol/L sodium hydroxide 50 μ L Stop the reaction, measure the absorbance value of each hole at 405nm, and judge the result of the tested sample.
(5) If it is a positive result, the sample can be further diluted to determine the antibody titer, compare the results of two consecutive samples, and judge

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Uncut Sheet Rapid Test Manufacturing Process

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